Identification of small molecule compounds with higher binding affinity to guanine deaminase (cypin) than guanine
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چکیده
منابع مشابه
Rabbit Liver Guanine Deaminase
Homogeneous guanine deaminase (guanine aminohydrolase, EC 3.5.4.3) is a single polypeptide chain of mean molecular weight of 55,000, as determined by Sephadex gel filtration and sodium dodecyl sulfate polyacrylamide disc gel electrophoresis. Irreversible inactivation was observed upon incubation with P-chloromercuribenzoate, at a concentration of 1.0 x lop4 M. Iodoacetic acid and iodoacetamide ...
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Using the human cDNA sequence corresponding to guanine deaminase, the Escherichia coli genome was scanned using the Basic Local Alignment Search Tool (BLAST), and a corresponding 439-residue open reading frame of unknown function was identified as having 36% identity to the human protein. The putative gene was amplified, subcloned into the pMAL-c2 vector, expressed, purified, and characterized ...
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ژورنال
عنوان ژورنال: Bioorganic & Medicinal Chemistry
سال: 2010
ISSN: 0968-0896
DOI: 10.1016/j.bmc.2010.07.054